Bone Marrow

print

Bone Marrow

BioCell


Bone marrow is the site of new blood cell production. It is rich in hematopoietic progenitor cells and mesenchymal stromal cells, as well as newly developed white blood cell populations. Many cell types can be isolated from collected bone marrow for use in research and clinical therapeutic applications.


0


Overview

Bone marrow is collected from both living and cadaveric donors via repeated iliac crest puncture into ACD or heparin anticoagulant. Bone marrow product is filtered through a 150µM filter to remove bone particulates. Bone marrow from living or cadaveric donor can be ordered as collected or additional processing can be performed to isolate specific cell populations or cryopreserve the product. Testing is available to enumerate cell types such as CD34+ cells, mesenchymal stromal cells, or lymphocytes in the product prior to further processing and shipping, as needed.

Technical Description PDF

To specify living or cadaveric product source, demand frequency, storage and shipping temperature, donor characteristics, additional processing, or testing needs, click on “Customize” tab.

Customize

BASIC REQUIREMENTS


Intention of Use
N/A RUO cGMP

Collection Method
N/A Aspirate

Regulatory Status
N/A IRB NDA BLA

Anticoagulant
N/A ACD

Cell Yield Target
N/A 1E6 1E9 3E6 3E9 5E9

Storage And Transport Temperature
N/A 1-10° C -18-20° C 20-24° C

Volume Needed
N/A 5ml 10ml 25ml 50ml 100ml

Per Week Demand
N/A 1-2 3-5 5-10 10-30

Duration of Order
N/A 1-3 Months 3-6 Months 6-12 Months 12-24 Months >24 Months




ADDITIONAL PROCESSING REQUESTS


Processing
N/A T-Cell Reduced Volume Reduced Aliquoted Wash Freeze Irradiated

HLA Typing Requirements
N/A Low Res Class 1 Low Res Class 2 High Res Class 1 High Res Class 2

Mesenchymal Enumeration
N/A CD14+ CD34- CD45- CD90+ CD73+ CD105+ HLA DR-

Age of Cells
N/A In-Date Fresh Expired

Lymphoid Enumeration
N/A CD3+ CD4+ CD8+ CD14+ CD16+ CD19+ HLA A2+




DONOR SPECIFICATIONS


Gender
N/A Male Female Both

Age
N/A 18-40 40-65 >65

Ethnicity
N/A Caucasian Native American African American Hispanic Asian Native Hawaiian or Pacific Islander

Blood Group / Type
N/A A+ A- B- B+ AB+ AB- O- O+

Criteria
N/A FACT AABB 21 CFR 600 21 CFR 1271 HRSA-OPTN

Preperation
N/A Fasting GCSF Mobilization Steriod Mobilization

Medications
N/A Have Taken Have Not Taken Cardiovascular Antihypertensives Penicillins Cephalosporins Sulfonamides Tetracyclines Antiarrhythmic Anticonvulsants Analgesics Immunologic Contraceptives

GI Agents Respiratory Metabolic Psychotherapeutic Hormones Sedatives Anticoagulants

Infectious Disease Test Positive
N/A HIV 1/2 HIV NAT HCV Antibody HCV NAT HBV Antibody HBV NAT WNV NAT Chagas Antibody Zika NAT CMV Antibody Syphilis Antibody

Abnormal Laboratory Test
N/A A1c AST ALT T4 TSH LDL HDL Total Cholesterol Pap Smear PSA

Cardiovascular Disease
N/A Congestive Heart Failure Stroke Angina Coronary Artery Disease High Blood Pressure Arrhythmia

Endocrine Disease
N/A Diabetes Type 2 Diabetes Type 1 Hyperthyroidism Hypothyroidism Hashimoto's Thryoiditis

Gynecological / Urological Disease
N/A Renal Insufficiency Chronic UTI Kidney Stones

Hematological Disease
N/A Anemia Leukemia Lymphoma Hemaphilia Sickle Cell TTP

Oncological Disease
N/A Liver Breast Prostate Cervical Lung Melanoma

Pulmonary Disease
N/A Asthma COPD Tuberculosis Cystic Fibrosis Emphysema

Autoimmune Disease
N/A Lupus Celiac Scleroderma Graves Rheumatoid Arthritis Sjögren's Addison's Chronic Thyroiditis

Digestive Disease
N/A Irritable Bowel Reflux Crohn's GERD Ulcer Ulcerative Colitis

Neurological Disease
N/A Migraines Seizures Alzheimer's Multiple Sclerosis

Ophthalmic Disease
N/A Glaucoma Cataracts Blindness Uveitis

COLLECT:

Custom collections from a pool of thousands of healthy donors.

CHARACTERIZE:

Flowcytometry characterization of immune cells.

ISOLATE:

Research or GMP grade positive or negative cell selection procedures.

EXPAND:

Flask or automated expansion services.

FREEZE:

Controlled rate freezing using custom cryo-preservatives.

STORE:

Monitored LN2 vapor phase storage of products and samples.