Mesenchymal Stromal Cells

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Mesenchymal Stromal Cells

BioCell


Mesenchymal stromal cells (MSCs) are multipotent stem cells that can differentiate into a variety of cell types, including osteoblasts (bone cells), chondrocytes (cartilage cells), myocytes (muscle cells) and adipocytes (fat cells). They can be isolated from various tissues in the body, including bone marrow, adipose tissue, dental pulp, placental tissue, and umbilical cord tissue. The multipotent properties of MSCs make them an ideal cell type for cellular therapies involving immunomodulation and regenerative medicine applications.


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Overview

Mesenchymal Stromal Cells (MSCs) are isolated from living and cadaveric bone marrow aspirate following protocols designed specifically for the isolation and growth of MSCs. Cells from each tissue product are characterized for their expression of CD73, CD90 and CD105 (>95%) and lacking expression of hematopoietic lineage markers CD34, CD45, CD11b, CD14, CD19 or CD79α, and HLA-DR. Each product source is also tested for their ability to differentiate into osteoblasts, chondrocytes, and adipocytes, as defined for MSCs by the International Society for Cellular Therapy (ISCT). Additional lineage marker analysis is available by request. Each MSC product is cryopreserved in vials at passages 2-4, and a specific passage is available upon request. Most experimental protocols utilize MSCs following the 4th passage.

To specify living or cadaveric MSC product source, desired passage requested, demand frequency, donor characteristics, and additional testing needs, click on “Customize” tab.

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BASIC REQUIREMENTS


Intention of Use
N/A RUO Clinical

Regulatory Status
N/A IRB NDA BLA

Per Week Demand
N/A 1-2 3-5 5-10 20-30 40-50 100

Storage and Transport Temperature
N/A 1-10 °C 20-24 °C -18 °C

Duration of Order
N/A 1-3 Months 4-6 Months 12 Months 24 Months Greater than 24 Months

Cell Yield Target
N/A 1E6 3E6 1E9 3E9 5E9

Anticoagulant
N/A CPD




ADDITIONAL PROCESSING REQUESTS


Processing
N/A Aliquoted Irradiated Freeze Wash Volume Reduced WBC Reduced HPC Reduced T-Cell Reduced Expansion

Lymphoid Enumeration
N/A CD3+ CD4+ CD8+ CD14+ CD16+ CD19+ HLA A2+

Mesenchymal Enumeration
N/A CD14+ CD34- CD45- CD73+ CD90+ CD105+ HLA DR-

Additional Testing Requirements
N/A Sickle-Trait Negative CMV Negative Serologic Phenotype DNA Phenotype

Viability
N/A 7-AAD Assay

HLA Typing Requirements
N/A Low Res Class 1 Low Res Class 2 High Res Class 1 High Res Class 2




DONOR SPECIFICATIONS


Gender
N/A Male Only Female Only Both

Age
N/A 18-40 50-65 Older than 65

Ethnicity
N/A Caucasian African American Native American Hispanic Asian Native Hawaiian or Pacific Islander

Criteria
N/A 21 CFR 1271 21 CFR 600 FACT HRSA-OPTN AABB

Preparation
N/A Passage 1 Passage 2 Passage 3 Passage 4

Blood Group / Type
N/A A+ A- B+ B- AB+ AB- O+ O-

Medications
N/A Have Taken Have Not Taken Cardiovascular Antiarrhythmic Penicillins Cephalosporins Sulfonamides Tetracyclines Antihypertensives Anticonvulsants Analgesics Sedatives Anticoagulants

GI Agents Hormones Immunologic Metabolic Psychotherapeutic Respiratory Contraceptives

Infectious Disease Test Positive
N/A HIV NAT HIV 1/2 HCV Antibody HCV NAT HBV Antibody HBV NAT WNV NAT Chagas Antibody Zika NAT CMV Antibody Syphilis Antibody

Abnormal Laboratory Test
N/A HDL Total Cholesterol LDL ALT AST PSA A1c T4 TSH Pap Smear

Cardiovascular Disease
N/A Congestive Heart Failure High Blood Pressure Coronary Artery Disease Angina Arrhythmia Stroke

Endocrine Disease
N/A Diabetes Type 1 Diabetes Type 2 Hypothyroidism Hyperthyroidism Hashimoto's Thyroiditis

Gynecological / Urological Disease
N/A Kidney Stones Chronic UTI Renal Insufficiency

Hematological Disease
N/A Hemophilia Sickle Cell Anemia TTP Lymphoma Leukemia

Oncological Disease
N/A Prostate Breast Cervical Liver Lung Melanoma

Pulmonary Disease
N/A COPD Asthma Cystic Fibrosis Emphysema Tuberculosis

Autoimmune Disease
N/A Rheumatoid Arthritis Addison's Chronic Thyroiditis Celiac Sjögren's Lupus Graves Scleroderma

Digestive Disease
N/A Crohn's Irritable Bowel GERD Ulcer Reflux Ulcerative Colitis

Neurological Disease
N/A Seizures Alzheimer's Multiple Sclerosis‎ Migraines

Ophthalmic Disease
N/A Blindness Cataracts Glaucoma Uveitis

COLLECT:

Custom collections from a pool of thousands of healthy donors.

CHARACTERIZE:

Flowcytometry characterization of immune cells.

ISOLATE:

Research or GMP grade positive or negative cell selection procedures.

EXPAND:

Flask or automated expansion services.

FREEZE:

Controlled rate freezing using custom cryo-preservatives.

STORE:

Monitored LN2 vapor phase storage of products and samples.