Buffy Coats

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Buffy Coats

BioCell


Whole blood-derived buffy coat products are rich in white blood cells and platelets. Buffy coats are a good source of cells for in vitro research applications. Due to the diversity of white blood cell populations in buffy coat products, further processing and characterization is typically required.


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Overview

Buffy Coat products are produced from 500 mL whole blood units collected using sodium citrate, dextrose, citric acid (CPD) anticoagulant. These products are red cell-reduced and contain leukocyte populations such as granulocytes, platelets, lymphocytes and other mononuclear cells. Each buffy coat is suspended in roughly 50 mL of plasma and contains approximately 500 million leukocytes/unit. Specific cell type isolation and cryopreservation from buffy coat products are available upon request.

Technical Description PDF

To specify demand frequency, storage and shipping temperature, donor characteristics, additional processing, or testing needs, click on “Customize” tab.

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BASIC REQUIREMENTS


Intention of Use
N/A RUO Clinical

Regulatory Status
N/A IRB NDA BLA

Collection Method
N/A Phlebotobmy

Cell Type
N/A MNC T-Cell B-Cell Platelet White Blood Cell

Volume
N/A 5ml 10ml 25ml 50ml

Per Week Demand
N/A 1-2 3-5 5-10 20-30 40-50 100 Greater than 100

Storage and Transport Temperature
N/A 1-10 °C 20-24 °C -18 °C

Duration of Order
N/A 1-3 Months 4-6 Months 12 Months 24 Months Greater than 24 Months




ADDITIONAL PROCESSING REQUEST


Anticoagulant
N/A ACD Heparin Citrate

Processing
N/A Aliquoted Freeze Wash Volume Reduced T-Cell Selection Ficoll Treated

Lymphoid Enumeration
N/A CD3+ CD4+ CD8+ CD14+ CD16+ CD19+ HLA A2+

HLA Typing Requirements
N/A Low Res Class 1 High Res Class 1 Low Res Class 2 High Res Class 2

Additional Testing Requirements
CMV N/A Sickle-Trait Negative




DONOR SPECIFICATIONS


Gender
N/A Female Only Male Only Both

Blood Group / Type
N/A A+ A- B+ B- AB+ AB- O+ O-

Age
N/A 18-40 50-65 Older than 65

Ethnicity
N/A African American Caucasian Native American Hispanic Asian Native Hawaiian or Pacific Islander

Criteria
N/A 21 CFR 600 21 CFR 1271 FACT HRSA-OPTN AABB

Preparation
N/A Fasting Steroid Mobilization GCSF Mobilization

Medications
N/A Have Not Taken Have Taken Cardiovascular Antiarrhythmic Penicillins Cephalosporins Sulfonamides Tetracyclines Antihypertensives Anticonvulsants Analgesics Sedatives Anticoagulants

GI Agents Hormones Immunologic Metabolic Psychotherapeutic Respiratory Contraceptives

Infectious Disease Test Positive
N/A HIV 1/2 HIV NAT HCV Antibody HCV NAT HBV Antibody HBV NAT WNV NAT Chagas Antibody Zika NAT CMV Antibody Syphilis Antibody

Abnormal Laboratory Test
N/A Total Cholesterol HDL LDL ALT AST PSA A1c T4 TSH Pap Smear

Cardiovascular Disease
N/A Congestive Heart Failure High Blood Pressure Coronary Artery Disease Angina Arrhythmia Stroke

Endocrine Disease
N/A Diabetes Type 1 Diabetes Type 2 Hyperthyroidism Hypothyroidism Hashimoto's Thyroiditis

Gynecological / Urological Disease
N/A Kidney Stones Chronic UTI Renal Insufficiency

Hematological Disease
N/A Sickle Cell Hemophilia Anemia TTP Lymphoma Leukemia

Oncological Disease
N/A Prostate Breast Cervical Liver Lung Melanoma

Pulmonary Disease
N/A COPD Asthma Cystic Fibrosis Emphysema Tuberculosis

Autoimmune Disease
N/A Rheumatoid Arthritis Addison's Chronic Thyroiditis Celiac Sjögren's Lupus Graves Scleroderma

Digestive Disease
N/A Irritable Bowel Crohn's GERD Ulcer Reflux Ulcerative Colitis

Neurological Disease
N/A Alzheimer's Seizures Multiple Sclerosis‎ Migraines

Ophthalmic Disease
N/A Blindness Cataracts Glaucoma Uveitis

COLLECT:

Custom collections from a pool of thousands of healthy donors.

CHARACTERIZE:

Flowcytometry characterization of immune cells.

ISOLATE:

Research or GMP grade positive or negative cell selection procedures.

EXPAND:

Flask or automated expansion services.

FREEZE:

Controlled rate freezing using custom cryo-preservatives.

STORE:

Monitored LN2 vapor phase storage of products and samples.