LEUKOCYTE REDUCTION SYSTEM (LRS) CONES

With over 150 cones analyzed, TNC content ranges from 1X10⁹ to 3X10⁹, with the average being 1.8X10⁹, with less than 5% granulocyte contamination. The RBC content of the chamber averages 5.6X10¹⁰ and the platelet content averages 2.0X10¹⁰ platelets.

When the cells from cones are eluted into plasma or cell culture media and stored at 2-6°C for up to 4 days, the cells remained >95% viable at 2 days and >90% viable at 4 days. When cones were stored uneluted at 2-6°C, cells remained 96.7% viable at 2 days and 92.5% viable at 4 days, on average.

T cells from LRSCs proliferated when stimulated with anti-CD3/CD28 beads or PHA and cultured in the presence of IL-2. CD14 monocytes from LRSCs differentiated into CD16 macrophages when cultured in a differentiation media cocktail containing insulin.

TNC 1.86X10^9

Monocytes 37.4%

Lymphocytes 57.4%

T Lymphocytes 34.0%

B Lymphocytes 6.0%

NK Cells 8.8%

LRS chambers are pooled to create a product with a standard TNC and CD3 count. Pooled products are ficoll treated and irradiated.