CliniMACS Cell Separation

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CliniMACS Cell Separation

BioLabs


The Miltenyi CliniMACS system uses monoclonal antibodies directed to specific “cluster of differentiation” antigens attached to ferrous micro-beads to isolate selected cells. The device uses an integrated tubing set to push cells through a magnetic chamber for controlled isolation.


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Overview

The Miltenyi CliniMACS system uses monoclonal antibodies directed to specific “cluster of differentiation” antigens attached to ferrous micro-beads to isolate selected cells. The device uses and integrated tubing set to push cells through a magnetic chamber for controlled isolation. Depending on the desired cell, either positive or negative cell selection can be achieved. The unique characteristics of the process ensures that micro-bead coated cells remain functional and usable in both clinical and research settings.

Using this semi-automated platform ensures batch-to-batch reproducibility of isolation methods. The method also allows use of serial isolations with a reduced chance of microbial contamination. Bio-Sharing works directly with the researcher to develop the isolation strategy needed to harvest cells and achieve the characteristics required in the cell population.

Customize

Source
N/A Buffy Coat Cord Blood Peripheral Blood Mobilized (Apheresis) Peripheral Blood Not Mobilized (LRS Chamber) Bone Marrow (Cadaveric)

Cell Type
N/A T-Lymphocytes T-Helper Lymphocytes T-Cytotoxic Lymphocytes B-Lymphocytes Mesenchymal Cells Dendritic Cells Hematopoietic Stem Cells

Isolation Method
N/A Positive Selection (CliniMACS) Negative Selection (CliniMACS)

Volume Needed
N/A 1 Million 2 Million 3 Million 4 Million 5 Million Greater than 5 Million

Storage
N/A Frozen Never Frozen

Transport Temperature
None 1-10 °C 20-24 °C -18 °C

HLA Type
N/A Typed Not Typed

COLLECT:

Custom collections from a pool of thousands of healthy donors.

CHARACTERIZE:

Flowcytometry characterization of immune cells.

ISOLATE:

Research or GMP grade positive or negative cell selection procedures.

EXPAND:

Flask or automated expansion services.

FREEZE:

Controlled rate freezing using custom cryo-preservatives.

STORE:

Monitored LN2 vapor phase storage of products and samples.