LRS "CONE" CHAMBERS

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LRS "CONE" CHAMBERS

BioCell


Leukoreduction system (LRS) chambers are filled with blood enriched with mononuclear cells and depleted of most granulocytes and platelets. LRS chambers are an excellent starting source to obtain monocyte and lymphocyte populations, especially T cells. Cells harvested from these chambers can be directly used in many in vitro research applications.


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Overview

During a routine platelet donation procedure, centrifugation separates mononuclear white blood cells from platelets, leaving the cells behind inside the LRS chamber. The cellular make-up and number of cells inside these chambers vary somewhat based on the specific collection procedure conducted. On average, these chambers have 1.7 billion mononuclear cells and <5% contaminating granulocytes. T cells can constitute up to 65% of the cells inside LRS chambers. Cells contained inside these chambers are sterile and remain viable for an extended period when stored at 2-8⁰C (>95% viable at 48 h and >90% viable at 96 h).

Technical Description PDF

To specify demand frequency, storage and shipping temperature, donor characteristics, additional processing, or testing needs, click on “Customize” tab.

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BASIC REQUIREMENTS


Intention of Use
N/A RUO

Regulatory Status
N/A IRB

Anticoagulant
N/A ACD

Storage And Transport Temperature
N/A -18-20° C 1-10° C

Duration of Order
N/A 1-3 Months 3-6 Months 6-12 Months 12-24 Months >24 Months

Cell Yield Target
N/A 1E6 3E6 1E9 3E9 5E9

Per Week Demand
N/A 1-2 3-5 5-10 10-30 30-50 50-100 >100




ADDITIONAL PROCESSING REQUESTS


Processing
N/A Volume Reduced Freeze Aliquoted Wash Ficoll Treated

HLA Typing Requirements
N/A High Res Class 1 High Res Class 2 Low Res Class 1 Low Res Class 2

HPC Enumeration
N/A CD34+

Viability
N/A 7-AAD Assay CFU Assay (CD34+, CD45+)

Mesenchymal Enumeration
N/A HLA DR- CD14+ CD34- CD45- CD73- CD90- CD105+

Lympoid Enumeration
N/A CD3+ CD4+ CD8+ CD16+ CD14+ CD19+ HLA A2+

Additional Testing Requirements
N/A HLA Typing Culture 7-AAD Assay




DONOR SPECIFICATIONS


Gender
N/A Male Female Both

Age
N/A 18-40 40-65 >65

Criteria
N/A AABB FACT 21 CFR 600 21 CFR 1271 HRSA-OPTN

Ethnicity
N/A Caucasian African American Native American Asian Native Hawaiian or Pacific Islander Hispanic

Preparation
N/A Steroid Mobilization GCSF Mobilization Fasting

Blood Group / Type
N/A A+ O- A- B+ O+ B- AB- AB+

Medications
N/A Sedatives Cephalosporins Sulfonamides Immunologic Tetracyclines Anticonvulsants GI Agents Analgesics Penicillins Metabolic Antihypertensives Cardiovascular Anticoagulants

Respiratory Hormones Aniarrhythmic Have Taken Psychotherapeutic Have Not Taken

Infectious Disease Test Positive
N/A WNV NAT HCV Antibody Zika NAT HBV Antibody Syphilis Antibody HBV NAT HCV NAT HIV 1/2 Chagas Antibody HIV NAT CMV Antibody

Abnormal Laboratory Test
N/A A1c Pap Smear PSA TSH T4 T4 ALT Total Cholestoral HDL AST LDL

Cardiovascular Disease
N/A Stroke Angina Arrhythmia Coronary Artery Disease Congestive Heart Failure High Blood Pressure

Endrocrine Disease
N/A Diabetes Type 1 Diabetes Type 2 Hypothyroidism Hyperthyroidism Hashimoto's Thyroiditis

Gynecological / Urological Disease
N/A Chronic UTI Kidney Stones Renal Insufficiency

Hematological Disease
N/A Leukemia Lymphoma Hemophilia TTP Sickle Cell Anemia

Oncological Disease
N/A Prostate Liver Melanoma Lung Breast Cervical

Pulmonary Disease
N/A Cystic Fibrosis Asthma Tuberculosis Emphysema COPD

Autoimmune Disease
N/A Celiac Addison's Chronic Thyroiditis Scleroderma Rheumatoid Arthritis Lupus Graves Sjögren's

Digestive Disease
N/A Irritable Bowel Reflux Ulcerative Colitis Crohn's GERD Ulcer

Neurological Disease
N/A Migraines Seizures Multiple Sclerosis Alzheimer's

Ophthalmic Disease
N/A Blindness Glaucoma Uveitis Cataracts

COLLECT:

Custom collections from a pool of thousands of healthy donors.

CHARACTERIZE:

Flowcytometry characterization of immune cells.

ISOLATE:

Research or GMP grade positive or negative cell selection procedures.

EXPAND:

Flask or automated expansion services.

FREEZE:

Controlled rate freezing using custom cryo-preservatives.

STORE:

Monitored LN2 vapor phase storage of products and samples.