Hematopoietic CFU Culture & Assay

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Hematopoietic CFU Culture & Assay

BioLabs


The Colony-Forming Unit (CFU) Assay is an in vitro culture process for enumerating multipotential and lineage-committed hematopoietic progenitor cells (HPCs) in bone marrow, blood and other hematopoietic tissues.


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Overview

To ensure viability of post processed cells, stem cells are cultured using methocult media and meniscus free smart dishes. After 14 days of tissue culture, the StemVision imaging and colony counting device is used to quantitate and classify the number of hematopoietic colony forming units produced by erythroid, myeloid and multipotent progenitor cells. The StemVision system acquires an image of the entire culture plate then uses advanced software to identify and classify each of the hematopoietic colonies. This software removes the CFU counting variability often seen using manual methods.

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Available CFU Culture and Assays
N/A HPC 14 Day CFU HPC 7 Day CFU Bone Marrow 7 Day CFU Bone Marrow 14 Days CFU Colony Counting and Imaging with CFU-E, BFU-E, CFU-G/M/GM and CFU-GEMM Analysis Report

COLLECT:

Custom collections from a pool of thousands of healthy donors.

CHARACTERIZE:

Flowcytometry characterization of immune cells.

ISOLATE:

Research or GMP grade positive or negative cell selection procedures.

EXPAND:

Flask or automated expansion services.

FREEZE:

Controlled rate freezing using custom cryo-preservatives.

STORE:

Monitored LN2 vapor phase storage of products and samples.