HEMATOPOIETIC PROGENITOR CELLS (HPC or HSC)

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HEMATOPOIETIC PROGENITOR CELLS (HPC or HSC)

BioCell


Hematopoietic progenitor cells are multipotent, self-renewing stem cells that give rise to all blood cells types: lymphoid, myeloid, and erythroid lineages. HPCs are found in and can be recovered from adult peripheral blood, adult bone marrow, and neonatal umbilical cord blood. HPCs collected from peripheral blood by apheresis are for research or clinical use.


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Overview

Hematopoietic progenitor cells are collected from GCSF-mobilized whole blood by apheresis. ACD anticoagulant is used at a ratio of 1:13, with a guarantee of at least 1.8 billion cells per product. However, HPC apheresis product may be collected to the specifications of the customer, such as a target number of cells or blood volume processed. In addition, if unique donor characteristics are specified, we are positioned to recruit the type of donors needed. Post-collection processing, HPC enumeration and/or isolation, and cryopreservation are additional services available upon request.

Technical Description PDF

CD34+ HPCs can be also isolated directly from bone marrow or cord blood products by custom request. Specify the desired source of HPCs on the “Customize” tab. In addition, specify demand frequency, storage and shipping temperature, donor characteristics, additional processing, or testing needs by clicking on “Customize” tab.

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BASIC REQUIREMENTS


Intention of Use
N/A RUO Clinical

Collection Method
N/A Apheresis

Regulatory Status
N/A IRB NDA BLA

Volume
N/A 5ml 10ml 25ml 50ml 100ml 200ml

Storage And Transport Temperature
N/A 1-10 °C -18-20 °C -190 °C

Per Week Demand
N/A 1-2 3-5 5-10 20-30 40-50 100 Greater than 100

Duration of Order
N/A 1-3 Months 4-6 Months 12 Months 24 Months Greater than 24 Months

Cell Yield Target
N/A 1E6 3E6 1E9 3E9 5E9

Anticoagulant
N/A CPD ACD Heparin




ADDITIONAL PROCESSING REQUESTS


Processing
N/A Aliquoted Freeze Wash Volume Reduced WBC Reduced T-Cell Reduced

Lymphoid Enumeration
N/A CD3+ CD4+ CD8+ CD14+ CD16+ CD19+ HLA A2+

Mesenchymal Enumeration
N/A CD14+ CD34- CD45- CD73+ CD90+ CD105+ HLA DR-

HPC Enumeration
N/A CD34+

Additional Testing Requirements
N/A CMV Negative CD34 Assay 7-AAD Assay HLA Typing Culture

HLA Typing Requirements
N/A Low Res Class 1 Low Res Class 2 High Res Class 1 High Res Class 2

Viability
N/A 7-AAD Assay CFU Assay (CD34+,CD45+)




DONOR SPECIFICATIONS


Gender
N/A Female Only Male Only Both

Age
N/A 18-40 50-65 Older than 65

Criteria
N/A 21 CFR 1271 21 CFR 600 FACT HRSA-OPTN AABB

Ethnicity
N/A Caucasian African American Native American Hispanic Asian Native Hawaiian or Pacific Islander

Blood Group / Type
N/A A+ A- B+ B- AB+ AB- O+ O-

Preparation
N/A Fasting Steroid Mobilization GCSF Mobilization

Medications
N/A Have Not Taken Have Taken Cardiovascular Antiarrhythmic Penicillins Cephalosporins Sulfonamides Tetracyclines Antihypertensives Anticonvulsants Analgesics Sedatives Anticoagulants

GI Agents Hormones Immunologic Metabolic Psychotherapeutic Respiratory Contraceptives

Infectious Disease Test Positive
N/A HIV 1/2 HIV NAT HCV Antibody HCV NAT HBV Antibody HBV NAT WNV NAT Chagas Antibody Zika NAT CMV Antibody Syphilis Antibody

Abnormal Laboratory Test
N/A HDL Total Cholesterol LDL ALT AST PSA A1c T4 TSH Pap Smear

Cardiovascular Disease
N/A Congestive Heart Failure High Blood Pressure Coronary Artery Disease Angina Arrhythmia Stroke

Endocrine Disease
N/A Diabetes Type 1 Diabetes Type 2 Hypothyroidism Hyperthyroidism Hashimoto's Thyroiditis

Gynecological / Urological Disease
N/A Chronic UTI Kidney Stones Renal Insufficiency

Hematological Disease
N/A Hemophilia Sickle Cell Anemia TTP Lymphoma Leukemia

Oncological Disease
N/A Breast Prostate Cervical Liver Lung Melanoma

Pulmonary Disease
N/A COPD Asthma Cystic Fibrosis Emphysema Tuberculosis

Autoimmune Disease
N/A Addison's Rheumatoid Arthritis Chronic Thyroiditis Celiac Sjögren's Lupus Graves Scleroderma

Digestive Disease
N/A Crohn's Irritable Bowel GERD Ulcer Reflux Ulcerative Colitis

Neurological Disease
N/A Seizures Alzheimer's Multiple Sclerosis‎ Migraines

Ophthalmic Disease
N/A Cataracts Blindness Glaucoma Uveitis

COLLECT:

Custom collections from a pool of thousands of healthy donors.

CHARACTERIZE:

Flowcytometry characterization of immune cells.

ISOLATE:

Research or GMP grade positive or negative cell selection procedures.

EXPAND:

Flask or automated expansion services.

FREEZE:

Controlled rate freezing using custom cryo-preservatives.

STORE:

Monitored LN2 vapor phase storage of products and samples.