Plasma is the pale yellow liquid portion of human blood. The primary purpose of plasma is to transport nutrients, hormones, and proteins and remove waste products from other cells. Plasma also provides the matrix for movement of all cellular (RBC, WBC, Platelets) elements of blood through the circulatory system.
Human Plasma samples and products are manufactured in a closed system by placing the whole blood units in a calibrated centrifuge. High RPM centrifugal force causes heavier cellular components to the bottom of the bag and the plasma with a density of 1.025 g/dl remains at the top of the centrifuged unit. Plasma provides the matrix that allows cellular blood component's to circulate through the vascular system. Water makes up greater than 90% of the volume of plasma, while dissolved proteins such as albumin, antibodies and fibrinogen make up 6-8% of the volume. Plasma also contains electrolytes, clotting factors, hormones and carbon dioxide excreted during cellular respiration. The viability of coagulation factors in plasma is affected by the elapsed time between phlebotomy and freezing and the elapsed time after thawing.Fresh Frozen Plasma is separated within 8 hours after phlebotomy and frozen to -18°C. After thawing, the Factor VIII activity is ≈ 107%, Factor II activity is ≈ 81%, Factor V activity is ≈ 79%, Factor VII activity is ≈ 90%, Factor X activity is ≈ 85%, and the fibrinogen content is ≈ 225 mg/dl. After having been thawed between 24-48 hours, the Factor VIII activity is significantly reduced. Other coagulation factors are relatively unchanged up to five days after thawing. Plasma can also be prepared with the cryoglobulin fraction (concentrated Factor VIII, Factor XIII, von Willibrand Factor, Fibronectin, and Fibrinogen) removed and the product refrozen.
Custom collections from a pool of thousands of healthy donors.
Flowcytometry characterization of immune cells.
Research or GMP grade positive or negative cell selection procedures.
Flask or automated expansion services.
Controlled rate freezing using custom cryo-preservatives.
Monitored LN2 vapor phase storage of products and samples.