Lymphocytes

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Lymphocytes

BioCell


There are three main types of lymphocytes central to innate and adaptive immunity: B cells, T cells, and NK cells. B cells produce antibodies in response to foreign invaders like bacteria and parasites. T cells facilitate a cytotoxic attack on infected or cancerous cells or provide help to B cells and other T cells. NK cells provide an innate response to infected or cancerous cells. Lymphocytes, especially T cells and NK cells, are the current focus of cutting-edge anti-cancer therapies.


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Overview

Purified lymphocyte populations can be obtained from multiple sources, upon request:



   Isolation from PBMC apheresis products: Donors with requested characteristics undergo automated PBMC apheresis to enrich for mononuclear cells in the blood. Those cells are further processed by magnetic-activated cell sorting (MACS) to obtain the requested cell type(s). Flow cytometry testing, functional testing, and cryopreservation are available upon request.

   Isolation from buffy coat products: Cells from a manufactured buffy coat undergoes Ficoll gradient centrifugation to enrich for mononuclear cells in the product. Those cells are further processed by magnetic-activated cell sorting (MACS) to obtain the requested cell type(s). Flow cytometry testing, functional testing, and cryopreservation are available upon request.

   Isolation from LRS chambers: LRS chambers will be salvaged from routine plateletpheresis procedures and the enriched mononuclear cells contained within will be harvested. Those cells are further processed by magnetic-activated cell sorting (MACS) to obtain the requested cell type(s). Flow cytometry testing, functional testing, and cryopreservation are available upon request.

   Isolation from bone marrow products: Bone marrow aspirate from living or cadaveric donor (as requested) undergoes Ficoll gradient centrifugation to enrich for mononuclear cells in the product. Those cells will be further processed by magnetic-activated cell sorting (MACS) to obtain the requested cell type(s). Flow cytometry testing, functional testing, and cryopreservation are available upon request.

To specify product source, desired purified cell type(s) needed, demand frequency, donor characteristics, and additional testing needs, click on “Customize” tab.

Customize

BASIC REQUIREMENTS


Intention of Use
N/A RUO Clinical

Regulatory Status
N/A IRB NDA BLA

Cell Type
N/A T-Cell B-Cell

Storage and Transport Temperature
N/A 1-10 °C 20-24 °C -18 °C

Volume
N/A 5ml 10ml 25ml 50ml 100ml 200ml

Per Week Demand
N/A 1-2 3-5 5-10 20-30 40-50 100 Greater than 100

Cell Yield Target
N/A 1E6 3E6 1E9 3E9 5E9

Duration of Order
N/A 1-3 Months 4-6 Months 12 Months 24 Months Greater than 24 Months

Anticoagulant
N/A CPD CP2D ACD Heparin




ADDITIONAL PROCESSING REQUESTS


Processing
N/A Aliquoted Freeze Wash Volume Reduced WBC Reduced Expansion

Lymphoid Enumeration
N/A CD3+ CD4+ CD8+ CD14+ CD16+ CD19+ HLA A2+

Additional Testing Requirements
N/A CMV Negative Sickle-Trait Negative Serologic Phenotype DNA Phenotype

Viability
N/A 7-AAD Assay




DONOR SPECIFICATIONS


Gender
N/A Male Only Female Only Both

Age
N/A 18-40 50-65 Older than 65

Ethnicity
N/A African American Caucasian Native American Hispanic Asian Native Hawaiian or Pacific Islander

Criteria
N/A 21 CFR 1271 21 CFR 600 FACT HRSA-OPTN AABB

Blood Group / Type
N/A A+ A- B+ B- AB+ AB- O+ O-

Preparation
N/A Steroid Mobilization Fasting GCSF Mobilization

Medications
N/A Have Taken Have Not Taken Cardiovascular Antiarrhythmic Penicillins Cephalosporins Sulfonamides Tetracyclines Antihypertensives Anticonvulsants Analgesics Sedatives Anticoagulants

GI Agents Hormones Immunologic Metabolic Psychotherapeutic Respiratory Contraceptives

Infectious Disease Test Positive
N/A HIV 1/2 HIV NAT HCV Antibody HCV NAT HBV Antibody HBV NAT WNV NAT Chagas Antibody Zika NAT CMV Antibody Syphilis Antibody

Abnormal Laboratory Test
N/A HDL Total Cholesterol LDL ALT AST PSA A1c T4 TSH Pap Smear

Cardiovascular Disease
N/A Congestive Heart Failure High Blood Pressure Coronary Artery Disease Angina Arrhythmia Stroke

Endocrine Disease
N/A Diabetes Type 1 Diabetes Type 2 Hypothyroidism Hyperthyroidism Hashimoto's Thyroiditis

Gynecological / Urological Disease
N/A Chronic UTI Kidney Stones Renal Insufficiency

Hematological Disease
N/A Sickle Cell Hemophilia Anemia TTP Lymphoma Leukemia

Oncological Disease
N/A Breast Prostate Cervical Liver Lung Melanoma

Pulmonary Disease
N/A Asthma COPD Cystic Fibrosis Emphysema Tuberculosis

Autoimmune Disease
N/A Addison's Rheumatoid Arthritis Chronic Thyroiditis Celiac Sjögren's Lupus Graves Scleroderma

Digestive Disease
N/A Irritable Bowel Crohn's GERD Ulcer Reflux Ulcerative Colitis

Neurological Disease
N/A Alzheimer's Seizures Multiple Sclerosis‎ Migraines

Ophthalmic Disease
N/A Cataracts Blindness Glaucoma Uveitis

COLLECT:

Custom collections from a pool of thousands of healthy donors.

CHARACTERIZE:

Flowcytometry characterization of immune cells.

ISOLATE:

Research or GMP grade positive or negative cell selection procedures.

EXPAND:

Flask or automated expansion services.

FREEZE:

Controlled rate freezing using custom cryo-preservatives.

STORE:

Monitored LN2 vapor phase storage of products and samples.